Authentication of Hybrid Purity in Eggplant (Solanum melongena L.) Through RAPD Analysis

Authentication of Hybrid Purity in Eggplant (Solanum melongena L.) Through RAPD Analysis

For testing seed purity and trueness, GOT (Grow Out Test) is a long term process and therefore, present study was carried out during Rabi-2011. The experimental materials used in the study comprised of four parents viz., Green Long (GL), Pusa Purple long (PPL), IBWL-2007-1 (IBWL), Punjab Sadabahar (PS) and their six F1’s and standard check PH-6. RAPD marker was used to test the hybridity. Out of twenty-nine, only four primers showed polymorphism. namely OPA -14, OPA-08, OPH-01 and OPH-20. According to banding pattern for the primer OPH-01, hybrid between P4 and P2 (IBWL-2007-1 X Green Long) resembles to male parent i.e., Green Long (P2) and in same reciprocal cross P2 and P4 (Green Long X IBWL-2007-1), the banding patterns of F1 hybrid resembles male parent i.e. IBWL-2007-1 (P4). In another hybrid, between P3 and P4 (Punjab Sadabahar X IBWL-2007-1) banding pattern of F1 hybrid resembles to male parent i.e., IBWL-2007-1 (P4). For the primer OPH-20, F1 hybrid between P1 and P3 (Punjab Sadabahar X IBWL-2007-1) banding pattern of F1 hybrid resembles to male parent i.e. IBWL-2007-1 (P3).For the primer OPA- 14, F1 hybrid between P3 and P4 (IBWL-2007-1 X Pusa Purple Long) banding pattern of F1 hybrid resembles to male parent i.e. Pusa Purple Long (P4) and in the same reciprocal cross P4 and P3 (Pusa Purple Long X IBWL-2007-1) banding pattern of F1 hybrid resembles to male parent i.e. IBWL-2007-1 (P3). For primer OPA-18, in F1 hybrid between P1 and P3 (Punjab Sadabahar X IBWL-2007-1), the banding pattern resembles to male parent i.e. IBWL-2007-1 (P3).