Research Article, J Vet Sci Med Diagn Vol: 3 Issue: 2
Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach
| Vaibhav D. Bhatt1, Anju P. Kunjadia2*, Keyur D. Bhatt3, Navin R. Sheth1 and Chaitanya G. Joshi4 | |
| 1Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360 005, Gujarat, India | |
| 2Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, ADIT campus, New Vallabh Vidyanagar -388 120, Anand, Gujarat, India | |
| 3Department of Chemistry, Gujarat University, Ahmedabad, Gujarat, India | |
| 4Department of Animal Biotechnology, College of Veterinary Science and A. H., Anand Agricultural University, Anand-388 001, Gujarat, India | |
| Corresponding author : Dr. Anju P. Kunjadia Associate Professor, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, ADIT campus, New Vallabh Vidyanagar -388 120, Anand, Gujarat, India E-mail: anjuaribas@gmail.com |
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| Received: February 06, 2014 Accepted: April 03, 2014 Published: April 08, 2014 | |
| Citation: Vaibhav DB, Anju PK, Keyur DB, Navin RS, Chaitanya GJ (2014) Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach. J Vet Sci Med Diagn 3:2. doi:10.4172/2325-9590.1000134 |
Abstract
Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach
Metagenomic analysis of milk samples collected from Gir, Kankrej (Bos indicus) and crossbred (Bos taurus X Bos indicus) cattle harbouring subclinical mastitis was carried out by pyrosequencing technology to elucidate virulence associated genes and sequences from the microbial community. Metabolic profile by a web-based tool MG-RAST revealed that members of Virulence, disease and defense associated genes were found to be 3.53,2.88 and 5.26 per cent (the percent values were calculated by finding its proportional hits among total hits obtained) in Gir, Kankrej and crossbred cattle, respectively.
