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Gus Kousoulas K

Gus Kousoulas K

Gus Kousoulas K, PhD
Division of Biotechnology & Molecular Medicine
Luisiana State University, USA

 

Biography

Dr. K. Gus Kousoulas received his BS in Physics from Fairleigh Dickinson University in Teaneck, NJ, and his MS and PhD degrees from Pennsylvania State University in Biophysics and Molecular Cell Biology, respectively. Dr. K. Gus Kousoulas received postdoctoral training at the University of Chicago working in Dr. Bernard Roizman’s laboratory and at the University of California at San Francisco with Dr. Lenore Pereira, where later he was promoted to Research Assistant Professor. Dr. K. Gus Kousoulas joined Louisiana State University in Baton Rouge, LA in 1988 and became full professor in 1994. Dr. K. Gus Kousoulas is currently Professor of Virology at the LSU School of Veterinary Medicine with adjunct appointments at the Department of Biological Sciences, College of Basic Sciences, the Department of Microbiology and Immunology at the LSU Health Sciences Center in New Orleans, and the LSU Health Sciences Center’s Gene Therapy Program and the Stanley S. Scott Cancer Center in New Orleans. He is also an affiliate member of the Tulane National Primate Research Center located in Covington, LA. Dr. Kousoulas has been independently funded by NIH with R01 grants since 1990 working on the molecular biology of herpes simplex virus. Dr. K. Gus Kousoulas is the Principal Investigator of the LSU-Tulane Center for Experimental Infectious Diseases, which is funded by the NIH:NCRR: COBRE mechanism and a mentor of a junior investigator in the LSU Health Sciences Center School of Dentistry COBRE (PI: Paul Fidel). Dr. Kousoulas is a member of the Steering Committee of the LSU Baton Rouge-led NCRR: INBRE program and leads the molecular and cellular biology core of the INBRE. Dr. Kousoulas has served on a number of NIH panels including the NCRR Comparative Medicine Panel and other NIH panels and site visit teams of National Primate Research Centers.

Research Interest

Dr. K. Gus Kousoulas primary research interests are focused on the molecular biology and pathogenesis of herpes simplex viruses (HSV) and human herpesvirus 8 (HHV-8) or Kaposi’s Sarcoma-Associated Herpesvirus (KSHV). Specifically, he seeks to define the molecular mechanisms that control attachment and penetration of these viruses into susceptible cells (including cells of neuronal origin for HSV), their ability to replicate and spread from cell to cell, and the role of membrane fusion events in intracellular virion transport and egress. His experimental approach utilizes advanced molecular biology, molecular genetics and cell biology. Briefly, mutant herpesviruses deficient in a particular function are isolated through generalized mutagenesis, site-specific mutagenesis of viral genomes cloned into bacterial artificial chromosomes (BAC). These engineered viruses are studied to learn about the role of specific virus encoded proteins in host cell attachment, penetration, virus induced cell fusion virion assembly and egress. To analyze the role of specific viral genes in virus penetration and virus-induced cell fusion these genes are expressed in transient, eukaryotic expression systems and the expressed proteins are detected using specific monoclonal antibodies produced in my laboratory. In addition, monoclonal and monospecific sera against viral proteins are utilized to locate their target proteins in infected cells using confocal and immunoelectron microscopy and to analyze their structure and function. A major finding from our studies has implicated multiple protein-protein interactions among viral membrane proteins of both HSV-1 and KSHV in regulation of virus-induced membrane fusion phenomena. Similarly, multiple interactions among membrane proteins and tegument proteins have been found to regulate cytoplasmic virion envelopment. A second major recent interest of his laboratory is the use of viral vectors for gene and ancer therapy and vaccine purposes. Currently funded projects include the generation of oncolytic recombinant herpes simplex virus vectors to combat breast cancer (Louisiana Board of Regents and Louisiana Gene Therapy and Cancer Consortia). These viruses are tested in zenograft mouse models using human breast cancer cells. A significant effort is focused on the production of vaccines for West Nile virus, herpes B virus and Simian Retrovirus using vesicular stomatitis virus (VSV) and adenovirus-based vectors. This work is carried out in collaboration with the Tulane National Research Primate Center (Dr. Preston Marx). A fourth research interest of Dr. K. Gus Kousoulas laboratory is the molecular biology and pathogenesis of human and bovine coronaviruses. Dr. K. Gus Kousoulas has derived and compared the entire genomic nucleotide sequence of several respiratory and enteric bovine coronaviruses and identified specific nucleotide changes, which may be associated with virus tropism. Dr. K. Gus Kousoulas is particularly interested in the structure and function of the viral spike glycoproteins encoded by the SARS coronavirus and respiratory bovine coronaviruses, which are known to promote virus entry into susceptible cells and virus-induced membrane fusion phenomena.

Publications

Chromosome 22q11.2 and 7q11.23 Microdeletions in Children with Congenital Heart Defects Detected by Fish Technique
Research Article:  J Genet Disor Genet Rep, 5:4
doi: 10.4172/2327-5790.1000142
Mirea AM, Popp RA, Lazea C, Cuzmici Z, Al Khzouz C, Bucerzan S, Nascu, Man S, Iurian S, Miclea DL
Abstract  |   Full-text  |   PDF  |  
Fetuin A Concentration in the Amniotic Fluid of Fetuses with Down Syndrome
Research Article:  J Genet Disor Genet Rep 2014, 4:1
doi: 10.4172/2327-5790.1000118
Salih Burcin Kavak, Ebru Celik Kavak,Askin Sen, Rasit Ilhan, Murat Kaya, Ekrem Sapmaz, ozgur Arat, Sel?uk Kaplan and Melike Baspinar
Abstract  |   Full-text  |   PDF  |  
Single Amino Acid Deletion in MYH11 Segregating in a Family with TAAD
Case Report:  J Genet Disor Genet Rep 2014, 4:1
doi: 10.4172/2327-5790.1000119
Pawel T Pomianowski, Daniel Dykas, Murim Choi, Jingshing Wu, Gregory A Kuzmik, Dawn Ardito, Sandip Mukherjee and John A Elefteriades
Abstract  |   Full-text  |   PDF  |  
Mutations in the RB1 Gene in Argentine Retinoblastoma Patients and Uncommon Clinical Presentations
Research Article:  J Genet Disor Genet Rep 2014, 4:1
doi: 10.4172/2327-5790.1000120
Ottaviani Daniela, Parma Diana, Ferrer Marcela, Giliberto Florencia, Luce Leonela, Alonso Cristina and Szijan Irene
Abstract  |   Full-text  |   PDF  |  
Impact of Consanguinity on Health in a Highly Endogamous Population in District Buner, Khyber Pakhtunkhwa, Pakistan
Research Article:  J Genet Disor Genet Rep 2014, 4:1
doi: 10.4172/2327-5790.1000121
Jehangir Khan, Arshad Ali, Bakht Tarin Khan , Zaheer Ahmad and Waqas Ahmad Shams
Abstract  |   Full-text  |   PDF  |  
Genetic Determinants of Salt- Sensitive Hypertension
Editorial:  J Genet Disor Genet Rep 2012, 1:1
doi: 10.4172/2327-5790.1000e101
Marlene Shehata, Fady Youssef and Alan Pater
Abstract  |   Full-text  |   PDF  |  
Genetic Studies of Complex Diseases in the Sequence Era
Editorial:  J Genet Disor Genet Rep 2012, 1:1
doi: 10.4172/2327-5790.1000e102
Momiao Xiong
Abstract  |   Full-text  |   PDF  |  
Translational Research to Uncover Diagnostic & Therapeutic Gene Targets Emerging in a Genomic Era: from Bench to Bedside
Editorial:  J Genet Disor Genet Rep 2012, 1:1
doi: 10.4172/2327-5790.1000e103
Blum K, Giordano J, Borsten J, Downs BW, Hauser M, Simpatico T, Lohmann R, Braverman ER and Barh D
Abstract  |   Full-text  |   PDF  |  
Genetic Networks in Heterogeneous Populations
Editorial:  J Genet Disor Genet Rep 2012, 1:1
doi: 10.4172/2327-5790.1000e104
Mogen Fenger
Abstract  |   Full-text  |   PDF  |  
CGH Array Based Case Report of a Patient Suffering with Amelogenesis Imperfecta, Jalili Syndrome, Situs Inversus and Oligozoospermia
Research Article:  J Genet Disor Genet Rep
doi: 10.4172/2327-5790.1000122
Neetu Singh, Dinesh Kumar Sahu, Parth Purwar, Sanjeev Gupta, Anil Kumar Tripathi, Jaya Dixit, Ravi Kant and Devendra Kumar Gupta
Abstract  |   Full-text  |   PDF  |  
RPOB Gene Polymorphism and its Association with Multi Drug Resistance Pattern of Mycobacterium Tuberculosis and Associated Risk Factors among TB Patients
Research Article:  J Genet Disor Genet Rep, 4:2
doi: 10.4172/2327-5790.1000123
Tekeba Sisay and Nega Berhane
Abstract  |   Full-text  |   PDF  |  
Ring14 International:Development of a National-Based Patient Association towards a “Global” Network Initiative to Fight a Chromosomal Disorder
Short Communication:  J Genet Disor Genet Rep, 4:2
doi: 10.4172/2327-5790.1000124
Stefania Azzali, Yssa DeWoody, Berardo Rinaldi and Marco Crimi
Abstract  |   Full-text  |   PDF  |  
Ring 9 Chromosome Syndrome in Black African Infant
Case Report:  J Genet Disor Genet Rep, 4:2
doi: 10.4172/2327-5790.1000125
Alao MJ, Lal�y� A, Adjagba M, Ayivi B and Darboux R
Abstract  |   Full-text  |   PDF  |  
Indigenous Complimentary Health Seeking Behavior among Caregivers of Sickle Cell Disorder in Nigeria
Research Article:  J Genet Disor Genet Rep, 4:2
doi: 10.4172/2327-5790.1000126
Omiunu Ojinga Gideon and Akahchukwu Patience Chioma
Abstract  |   Full-text  |   PDF  |  
History Versus Limits of Science: Is Solomonic Genius a Y Chromosome Phenomenon?
Rapid Communication:  J Genet Disor Genet Rep 2014, 3:2
doi: 10.4172/2327-5790.1000114
F I D Konotey-Ahulu
Abstract  |   Full-text  |   PDF  |  
Maternal and Paternal Age at Pregnancy for Low Incidence Trisomy Groups: Preliminary Findings and Implications
Research Article:  J Genet Disor Genet Rep 2014, 3:2
doi: 10.4172/2327-5790.1000115
Katherine S Ancell and Deborah A Bruns
Abstract  |   Full-text  |   PDF  |  
Cross-Sectional Cohort Ukrainian Megapolice Sample Study of People�s Reactions to Faces of Patients with Different Mental Conditions
Research Article:  J Genet Disor Genet Rep 2014, 3:2
doi: 10.4172/2327-5790.1000116
Filiptsova OV and Atramentova LA
Abstract  |   Full-text  |   PDF  |  
Molecular Genetic Testing for Carrier - Prenatal Diagnosis and Computational Analysis of Oculocutaneous Albinism Type 1
Research Article:  J Genet Disor Genet Rep 2014, 3:2
doi: 10.4172/2327-5790.1000117
Kathirvel Renugadevi, John Asnet Mary, Vijayalakshmi Perumalsamy, Suresh Seshadri, Sujatha Jagadeesh, Beena Suresh, Sheela Nampoothiri, Rajaiah Shenbagarathai, Sankaran Krishnaswamy and Periasamy Sundaresan
Abstract  |   Full-text  |   PDF  |   Supplementary File | 
Homozygous Point Mutation in a Patient with Spinal Muscular Atrophy Type 1
Case Report:  J Genet Disor Genet Rep 2016, 5:3
doi: 10.4172/2327-5790.1000137
Isa Abdi Rad, Ali Vahabi and Ahad Ghazavi
Abstract  |   Full-text  |   PDF  |  
Severe Expressive-Language Delay and Congenital Malformations in a Boy with Microduplication 7q11.23 Diagnosed by Molecular Cytogenetic Analysis
Research Article:  J Genet Disor Genet Rep 2016, 5:1
doi: 10.4172/2327-5790.1000127
Inesse Ben-Abdallah-Bouhjar, Amal Al Hashem, Samia Sobki, Brahim Tabarki, Yasser Hassen Babair and Hatem Elghezal
Abstract  |   Full-text  |   PDF  |  
Mutational Analysis in Gaucher Disease: Implications in Genetic Counseling and Management.
Research Article:  J Genet Disor Genet Rep 2016, 5:2
doi: 10.4172/2327-5790.1000132
Inusha Panigrahi, Jaswinder Kalra, Prasoon Goyad, Preeti Khetarpal and Anjana Munshi
Abstract  |   Full-text  |   PDF  |  
Treatment with Agalsidase Alfa during Pregnancy in a Heterozygous Female with Fabry Disease
Case Report:  J Genet Disor Genet Rep, 5:4
doi: 10.4172/2327-5790.1000143
Antonio Pisani, Giuseppe Bifulco, Attilio Di Spiezio Sardo and Eleonora Riccio
Abstract  |   Full-text  |   PDF  |  
Evidence that lithium Inhibits Export of N-Acetyl-L-Aspartate from Neurons: A Retrospective Study of Canavan Disease and Bipolar Disorder Patients
Research Article:  J Genet Disor Genet Rep 2014, 3:1
doi: 10.4172/2327-5790.1000110
Morris H Baslow and David N Guilfoyle
Abstract  |   Full-text  |   PDF  |  
Hereditary Thrombocytosis in 3 Kuwaiti Siblings with Homozygous MPL Pro106Leu Mutation and Abnormal Platelet Aggregation
Case Report:  J Genet Disor Genet Rep 2013, 2:2
doi: 10.4172/2327-5790.1000105
Preethi Reddy Marri, Salman Kirmani and Vilmarie Rodriguez
Abstract  |   Full-text  |   PDF  |  
Inherited Unbalanced Chromosome from Parent with Balanced Translocation: A Case Report and Review of Literature
Case Report:  J Genet Disor Genet Rep 2016, 5:3
doi: 10.4172/2327-5790.1000138
Kumari P, Mishra VV and Tewari S
Abstract  |   Full-text  |   PDF  |  
Screening of Genetic Mutations in GBA1, GIGYF2 and VPS35 in Parkinson Disease Patients from India
Research Article:  J Genet Disor Genet Rep, 5:4
doi: 10.4172/2327-5790.1000144
Tamali Halder, Janak Raj, Sharad Pandey, Ajay Kumar, Sagar Kawale, Sandeep Chaudhary, Vivek Sharma, Deepika Joshi and Parimal Das
Abstract  |   Full-text  |   PDF  |  
The Genomic Novel and Priority Mapping Tool: Using Empathic Design to Develop Innovative Patient-Centered Decision-Making Tools for the Genomic Testing Experience
Research Article:  J Genet Disor Genet Rep 2016, 5:1
doi: 10.4172/2327-5790.1000128
Kiley J Johnson, Kimberly A Schahl, Pamela S Sinicrope, Tammy M McAllister, Jennifer B McCormick, Leslie E Ruckman, Mekayla I Beaver and Noralane M Lindor
Abstract  |   Full-text  |   PDF  |  
Comprehensive Review of Rare Hereditary Autoinflammatory Disorders
Review Article:  J Genet Disor Genet Rep 2013, 2:2
doi: 10.4172/2327-5790.1000106
Nobuo Kanazawa
Abstract  |   Full-text  |   PDF  |  
Growth Abnormalities Resulting in Short Stature in Genetic Syndromes
Review Article:  J Genet Disor Genet Rep 2014, 3:1
doi: 10.4172/2327-5790.1000111
Lena Dain1 and Stavit A Shalev
Abstract  |   Full-text  |   PDF  |  
A Case of Wolf-Hirschhorn Syndrome and Familial Mediterranean Fever
Case Report:  J Genet Disor Genet Rep 2016, 5:2
doi: 10.4172/2327-5790.1000133
Kazuaki Matsumoto* and Masayasu Ohta
Abstract  |   Full-text  |   PDF  |  
Are Antimullerian Hormone and its Receptor Genes Associated with Low Ovarian Response?
Research Article:  J Genet Disor Genet Rep 2014, 3:1
doi: 10.4172/2327-5790.1000112
Chelsi Goodman, Hosam Zaki, Larry Fischel, Hisham Greiss and Carolyn Coulam
Abstract  |   Full-text  |   PDF  |  
Evaluation of Genomic Evidence for Oxidative Stress in Experimental Radiation Nephropathy
Research Article:  J Genet Disor Genet Rep 2013, 2:1
doi: 10.4172/2327-5790.1000101
Eric P Cohen, Marek Lenarczyk, Brian L Fish, Shuang Jia, Martin J Hessner and John E Moulder
Abstract  |   Full-text  |   PDF  |  
Left Ventricular Noncompaction in Noonan Syndrome
Case Report:  J Genet Disor Genet Rep 2016, 5:2
doi: 10.4172/2327-5790.1000134
Robert B Hinton, Paula Goldenberg, Richard C Godby, Ashley Parrott, Amy G Shikany, Benjamin J Landis, Jeanne F James, Erin M Miller, Stephanie M Ware
Abstract  |   Full-text  |   PDF  |  
Anti-Ige (Omalizumab) Improved Trombotic Emboli by Elevating Activated Protein C, Protein S, and Antithrombin III in a Case of Prothrombin G20210A Mutation: Long Term Follow-Up
Letter to Editor:  J Genet Disor Genet Rep 2016, 5:1
doi: 10.4172/2327-5790.1000129
Arzu Didem Yalcin and Betul Celik
Abstract  |   Full-text  |   PDF  |  
Congenital Cerebral Ischemic Lesions in Monochorionic Twins
Case Report:  J Genet Disor Genet Rep 2013, 2:2
doi: 10.4172/2327-5790.1000107
Olajide Dolapo and Ravi Kudumula
Abstract  |   Full-text  |   PDF  |  
Clinical and Molecular Assessment in a Female with Fragile X Syndrome and Tuberous Sclerosis
Research Article:  J Genet Disor Genet Rep 2016, 5:3
doi: 10.4172/2327-5790.1000139
Carolyn M Yrigollen, Laura Pacini, Veronica Nobile, Reymundo Lozano, Randi J Hagerman, Claudia Bagni and Flora Tassone
Abstract  |   Full-text  |   PDF  |  
Smith-Magenis Syndrome Treated with Ramelteon and Amphetamine-dextroamphetamine: Case Report and Review of the Literature
Case Report:  J Genet Disor Genet Rep, 5:4
doi: 10.4172/2327-5790.1000145
Baek WS, Elsea SH
Abstract  |   Full-text  |   PDF  |  
Long-Term Follow-Up of a Patient with Sitosterolemia and Hemolytic Anemia with Excellent Response to Ezetimibe
Case Report:  J Genet Disor Genet Rep 2013, 2:1
doi: 10.4172/2327-5790.1000102
Alfonso Quint?s-Cardama and John J. McCarthy
Abstract  |   Full-text  |   PDF  |  
Same Mutation in Two Patients with Mucopolysaccharidosis Type VI (Maroteaux-Lamy Syndrome) Coming from Different Municipalities in the Department of Cauca, Southwestern Colombia
Research Article:  J Genet Disor Genet Rep, 5:4
doi: 10.4172/2327-5790.1000146
Acosta MA, Lago RM, Barros F and Carracedo AM
Abstract  |   Full-text  |   PDF  |  
Lipoprotein Glomerulopathy: Molecular Characterization of Three Italian Patients and Literature Survey
Research Article:  J Genet Disor Genet Rep 2014, 3:1
doi: 10.4172/2327-5790.1000113
Antonio Pasquariello, Livia Pisciotta, Tiziana Sampietro, Giovanna Pasquariello, Pellegrino Masiello, Matilde Masini, Francesco Sbrana, Mariarita Puntoni, Roberto Miccoli, Sebastiano Calandra and Stefano Bertolini
Abstract  |   Full-text  |   PDF  |   Supplementary File | 
Spectrum of CFTR Mutations in the Algerian Population: Molecular and Computational Analysis
Research Article:  J Genet Disor Genet Rep 2016, 5:1
doi: 10.4172/2327-5790.1000130
Fatima Zohra Sediki, Abdelkarim Radoui, Abdallah Boudjema, Meriem Abdi, Faouzia Zemani-Fodil, Nadhira Saidi-Mehtar and Faiza Cabet
Abstract  |   Full-text  |   PDF  |  
Phenylketonuria (PKU) - What Next? Mini-Review
Review Article:  J Genet Disor Genet Rep 2013, 2:2
doi: 10.4172/2327-5790.1000108
WB Hanley
Abstract  |   Full-text  |   PDF  |  
Cerebrovascular and Brain Abnormalities in Autosomal- Dominant Polycystic Kidney Disease: Role of 3d Time-of-Flight Magnetic Resonance Angiography
Research Article:  J Genet Disor Genet Rep 2016, 5:3
doi: 10.4172/2327-5790.1000140
Carmela Russo, Ferdinando Caranci, Massimo Imbriaco, Manuela Napoli, Antonio Pisani, Eleonora Riccio, Michele Santangelo, Sirio Cocozza, Enrico Tedeschi, Francesco Briganti and Arturo Brunetti
Abstract  |   Full-text  |   PDF  |  
Variation of Abnormal Hemoglobins Concentrated in Durg, Chhattisgarh: A Brief Note Based on Cross-Sectional Study
Case Report:  J Genet Disor Genet Rep 2016, 5:2
doi: 10.4172/2327-5790.1000135
Devendra Lingojwar, Pramod Gupta, Savita Bhutoria, Sarita Lingojwar, Nikhil Mishra, Anil Kumar
Abstract  |   Full-text  |   PDF  |  
Erring on the Side of Life: Children with Rare Trisomy Conditions, Medical Interventions and Quality of Life
Review Article:  J Genet Disor Genet Rep 2013, 2:1
doi: 10.4172/2327-5790.1000103
Deborah A. Bruns
Abstract  |   Full-text  |   PDF  |  
Telomeres in Cancer: Length, Positioning and Epigenetics
Review Article:  J Genet Disor Genet Rep 2016, 5:1
doi: 10.4172/2327-5790.1000131
Patel TN, Sulekha R Nair, Lekshmi Mohan, Fahmina Y, Ashwini, Devi S and VA Saimadhukiran Dabbiru
Abstract  |   Full-text  |   PDF  |  
Genotype and Allele Frequencies of Calcium-Sensing Receptor Gene a986s (rs1801725) Polymorphism in Saudi Adults
Research Article:  J Genet Disor Genet Rep 2016, 5:3
doi: 10.4172/2327-5790.1000141
Sonbol HS and Al Otaibi WF
Abstract  |   Full-text  |   PDF  |  
Genotype-phenotype characteristics of β thalassemia children in the Gaza Strip, Palestine
Research Article:  J Genet Disor Genet Rep 2013, 2:2
doi: 10.4172/2327-5790.1000109
Maged M Yassin, Mahmoud M Sirdah, Rami M Al Haddad, Abdel-Monem H. Lubbad and Mansour S Al-Yazji
Abstract  |   Full-text  |   PDF  |  
Cleidocranial Dysplasia in a Mother and her New-born Daughter
Case Report:  J Genet Disor Genet Rep 2016, 5:2
doi: 10.4172/2327-5790.1000136
Ma?gorzata Napieralska, Aleksandra Modlinska, Tomasz Rybkiewicz, Regina ?uralska
Abstract  |   Full-text  |   PDF  |  
Mini Review: HLA B27 and its Immunogenetics in Ankylosing Spondylitis
Review Article:  J Genet Disor Genet Rep 2013, 2:1
doi: 10.4172/2327-5790.1000104
Hamid Nawaz Tipu
Abstract  |   Full-text  |   PDF  |  
 
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