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Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Jian-Cong Lin, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Ming Li, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Wen-Ming Xu, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Bo Peng, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Ze-Long Guo, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Wei Shui, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Yan-li Xin and Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China Chang-Ran Zhang*
 
Corresponding author : Chang-Ran Zhang, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China, Tel: 86-20-82379627;
Fax:
86-20-82376480; E-mail: zhcr2303@sina.com.cn
 
Received: June 17, 2013 Accepted: August 05, 2013 Published: August 09, 2013
 
Citation: Lin JC, Li M, Xu WM, Peng B, Guo ZL, et al. (2013) Identification of Unique Volatile Compounds of Aspergillus fumigatus for Potential Diagnostic Breath Test by HSSPME and GC-MS. J Immunol Tech Infect Dis 2:3. doi:10.4172/2329-9541.1000112
 
Abstract
 
The 13/14C-urea breath test has been used in the diagnosis of gastric helicobacter pylori. The use of breath tests for diagnosis has been applied over the last 20 years. However, its applicability for diagnosis of pulmonary fungus infection was less studied. In this study, Aspergillus fumigatus-specific volatile compounds were qualitatively analyzed by headspace solid phase microextraction (HS-SPME) and gas chromatography/mass spectrometry (GC/ MS). Klebsiella pneumoniae, Streptococcus pneumoniae, and Candida albicans, common nosocomial infection strains were used as controls. Results showed that 7,11-dimethyl-3-methylene- 1,6,10-dodecatriene were Aspergillus fumigatus-specific volatile compounds, which were not detected in the volatile metabolism products from bacteria or other fungi, and these pneumonia patients which were infected with Klebsiella pneumoniae, Streptococcus pneumoniae and Pseudomonas aeruginosa. Six patients which were infected with Aspergillus fumigatus diagnosed by histological biopsy, indicated that 7,11-dimethyl-3-methylene- 1,6,10-dodecatriene may be biochemical indicators for Aspergillus fumigatus infection. The use of a breath test to detect fungispecific volatile compounds may have the potential value of being a diagnostic indicator of pulmonary fungus infection.
 
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