Research Article, J Biocatal Biotransformation Vol: 2 Issue: 1
Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis
| Jun Cai1*, Jin Li2, Changgao Wang1, Jianguo Lin1, Ying Hu1, Jianhong Yang3, Yumin Du4 and Hua Zheng5 | |
| 1Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China | |
| 2College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | |
| 3Department of Environmental Engineering, School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China | |
| 4Department of Environmental Science, College of Resource and Environmental Science, Wuhan University, Wuhan 430079, Wuhan, China | |
| 5Department of Pharmaceutical Engineering, School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China | |
| Corresponding author : Jun Cai Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China Tel: +86-27-88032319; Fax: +86-27-88032320 E-mail: hgcaijun@126.com |
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| Received: February 04, 2013 Accepted: February 22, 2013 Published: February 28, 2013 | |
| Citation: Cai J, Li J, Wang C, Lin J, Hu Y, et al. (2013) Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis. J Biocatal Biotransformation 2:1. doi:10.4172/2324-9099.1000103 |
Abstract
Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis
The culture medium, fermentation conditions and effects of different inorganic salts on producing extracellular chitin deacetylase by Scopulariopsis brevicaulis were optimized.The highest enzymatic activity of deacetylation of chitin was 36 units/mL for optimum culture medium which included 2% (w/v) 3,6-O-carboxymethylchitin, 1% sucrose, 0.2% NaNO3, 0.1% K2HPO4, 0.05% KCl, 0.4% peptone, 0.05% MgSO4, 0.05% MnSO4, 0.03% CoCl2 and optimum cultural conditions included temperature, pH, agitation speed, fermenting time and volume and were 29°C, 7.0, 200 rmp min-1, 96 hours and 80 mL in 300 mL Erlenmeyer flask, respectively