GET THE APP

Utilization of Antagonistic Yeasts in Grapes to Control Botrytis Cinerea

Journal of Food and Nutritional Disorders.ISSN: 2324-9323

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Utilization of Antagonistic Yeasts in Grapes to Control Botrytis Cinerea

There is a growing demand for biopreservatives that are natural and eco-friendly. Accordingly, yeasts are considered to be one of the promising biocontrol agents. In addition, the use of chemical pesticides may lead to health problems by inducing resistance in the fruit microbiome, which also poses a threat to theenvironment. Therefore, in this study, Metschnikowia sp. (Metschnikowia sinensis KTMD1-4, Metschnikowia pulcherrima BMD3B, and Metschnikowia aff. pulcherrima P01A016) yeasts were tested on grapes to control Botrytis cinerea infection. For all yeasts, in vitro mycelial growth inhibition rates were found above 80 %. Additional tests (iron depletion, volatile organic compounds (VOCs), and biofilm activity) were conducted on yeasts to elucidate the inhibition mechanisms. Then, antagonistic yeasts were tested on grapes to see the biocontrol efficacy against B. cinerea. The findings showed that M. pulcherrima BMD3B had the highest iron depletion activity and biocontrol efficacy in controlling B. cinerea, also maintaining the integrity of the fruit cell wall. Furthermore, M. sinensis KTMD1-4 had a moderate effect in controlling B. cinerea. Although Metschnikowia aff. pulcherrima P01A016 displayed the highest biofilm activity (0.88 ± 0.03) and VOCs formation, it was ineffective in controlling B. cinerea. Metschnikowia yeasts prolonged the shelf life of grapes by reducing the disease incidence and lesion diameters compared Metschnikowia pulcherrima BMD3B, and Metschnikowia aff. pulcherrima P01A016) yeasts were tested on grapes to control Botrytis cinerea infection. For all yeasts, in vitro mycelial growth inhibition rates were found above 80 %. Additional tests (iron depletion, volatile organic compounds (VOCs), and biofilm activity) were conducted on yeasts to elucidate the inhibition mechanisms. Then, antagonistic yeasts were tested on grapes to see the biocontrol efficacy against B. cinerea. The findings showed that M. pulcherrima BMD3B had the highest iron depletion activity and biocontrol efficacy in controlling B. cinerea, also maintaining the integrity of the fruit cell wall. Furthermore, M. sinensis KTMD1-4 had a moderate effect in controlling B. cinerea. Although Metschnikowia aff. pulcherrima P01A016 displayed the highest biofilm activity (0.88 ± 0.03) and VOCs formation, it was ineffective in controlling B. cinerea. Metschnikowia yeasts prolonged the shelf life of grapes by reducing the disease incidence and lesion diameters compared to control. These findings could pave the way for the use of natural yeasts as an alternative to synthetic pesticides.

Special Features

Full Text

View

Track Your Manuscript

Media Partners

Associations