Figure 3: Binding analyses of miR-19a and miR-126 to the 3’UTR of F3. Depicted are the results of the dual luciferase reporter assay. In (A) the influence of the miR-19a hairpin inhibitor (anti-miR-19a) on the relative luciferase activity of the miTargetTM miRNA 3’UTR target clone pEZXMT01, containing the 3’UTR of F3 (3’UTR F3) is shown in HMEC-1 24 h post transfection. As controls, cells were co-transfected with a negative control vector (3’UTR control) and anti-miR-19a or a non-inhibitory negative hairpin miRNA (anti-miR-control), respectively. In (B) the impact of the miR- 126 hairpin inhibitor (anti-miR-126) on the luciferase activity was analyzed in analogous experimental settings. Renilla luciferase activity was normalized to firefly luciferase activity. (*) p<0.05; (n.s.) no significant difference compared to control; n≥4.