| |
Primer code/ Locus |
Gene sequence (51-31) |
No. of bands |
Polymorphic bands |
% |
*PIC |
*MI |
| |
|
ISSR |
|
|
|
|
|
| 1 |
R-7 |
CCCGGATCCCACACACACACACACACA |
10 |
8 |
80.0 |
0.321 |
2.568 |
| 2 |
UBC 817 |
CACACACACACACACAA |
9 |
7 |
77.7 |
0.265 |
1.852 |
| 3 |
UBC 825 |
GTGTGTGTGTGTGTGTA |
8 |
7 |
87.5 |
0.166 |
1.159 |
| 4 |
UBC 827 |
GTGTGTGTGTGTGTGTT |
10 |
8 |
80.0 |
0.300 |
2.401 |
| 5 |
UBC 834 |
TGTGTGTGTGTGTGTGA |
9 |
7 |
77.7 |
0.276 |
1.934 |
| 6 |
UBC 841 |
GAGAGAGAGAGAGAGAYC |
10 |
9 |
90.0 |
0.318 |
2.859 |
| 7 |
UBC 855 |
GTGTGTGTGTGTGTGTYA |
6 |
5 |
83.3 |
0.234 |
1.171 |
| 8 |
UBC 856 |
GTGTGTGTGTGTGTGTYC |
12 |
12 |
100 |
0.355 |
4.264 |
| 9 |
UBC 857 |
GTGTGTGTGTGTGTGTYG |
8 |
8 |
100 |
0.333 |
2.666 |
| 10 |
UBC 865 |
CCGCCGCCGCCGCCGCCG |
6 |
6 |
100 |
0.237 |
1.420 |
| 11 |
|
Total/Mean |
88 |
77 |
87.5 |
0.281 |
2.229 |
| |
|
RAPD |
|
|
|
|
|
| 12 |
OPM-15 |
GACCTACCAC |
5 |
4 |
80.0 |
0.187 |
0.748 |
| 13 |
OPM-20 |
AGGTCTTGGG |
7 |
6 |
85.7 |
0.119 |
0.714 |
| 14 |
OPO-06 |
CCACGGGAAG |
10 |
8 |
80.0 |
0.206 |
1.648 |
| 15 |
OPP-10 |
TCCCGCCTAC |
8 |
6 |
75.0 |
0.252 |
1.512 |
| 16 |
OPN-05 |
ACTGAACGCC |
6 |
4 |
66.7 |
0.165 |
0.660 |
| 17 |
OPO-03 |
CTGTTGCTAC |
8 |
3 |
37.5 |
0.183 |
0.549 |
| 18 |
OPN-11 |
TCGCCGCAAA |
11 |
2 |
18.2 |
0.074 |
0.148 |
| 19 |
OPO-02 |
ACGTAGCGTC |
4 |
4 |
100.0 |
0.367 |
1.468 |
| 20 |
OPM-18 |
CACCATCCGT |
4 |
3 |
75.0 |
0.265 |
0.795 |
| 21 |
OPO-01 |
GGCACGTAAG |
12 |
12 |
100 |
0.366 |
4.392 |
| 22 |
|
Total/Mean |
75 |
52 |
71.8 |
0.218 |
1.263 |
| |
|
SSR |
|
|
|
|
|
| 23 |
CS-1
mAoR2 |
F-GGCCATGGGAAACAACAA
R-GGAAGGGCATTATGGGTAAG |
1 |
1 |
100 |
0.278 |
0.278 |
| 24 |
CS-2
mAoR3a) |
F-CAGAACCGTCACTCCACTCC
R-ATCCAGACGAAGAAGCGATG |
2 |
1 |
50 |
0.214 |
0.214 |
| 25 |
CS-3
mAoR6c |
F-CAAAACTAGCCGGAATCTAGC
R-CCCCATCAAACCCTTATGAC |
2 |
2 |
100 |
0.411 |
0.822 |
| 26 |
CS-4
mAoR7b |
F-AACCTTCACTCCTCTGAAGC
R-GTGAATCCAAAGCGTGTG |
2 |
2 |
100 |
0.463 |
0.926 |
| 27 |
CS-5
mAoR11c |
F-ATCCAACAGCCACAATCCTC
R-CTTACAGCCCCAAACTCTCG |
2 |
2 |
100 |
0.209 |
0.418 |
| 28 |
CS-7
mAoR12 |
F-TCACCAAGATTGTGCTCCTG
R-AAACTACGTCCGGTCACACA |
2 |
2 |
100 |
0.293 |
0.586 |
| 29 |
CS-8
mAoR16c |
F-GGAGAAAGCAGTGGAGTTGC
R-CAAGTGAGTCCTCTCACTCTCA |
3 |
3 |
100 |
0.404 |
1.212 |
| 30 |
CS-14
mAoR17b |
F-GCAATGTGCAGACATGGTC
R-GGTTTCGCATGGAAGAAGAG |
3 |
3 |
100 |
0.445 |
1.335 |
| 31 |
CS-18
mAoR26 |
F-TCCACAAAATCAGCCTCCAC
R-GAGCGCTCGTGTCCTGTACT |
4 |
4 |
100 |
0.452 |
1.808 |
| 32 |
CS-13
mAoR29c |
F-GGAGAAGAAAAGTTAGGTTTGAC
R-CGTCTTCTTCCACATGCTTC |
3 |
3 |
100 |
0.348 |
1.044 |
| 33 |
CS-15
mAoR33 |
F-CATCCTTTTGCCAATTAAAAACA
R-CACGTGTATTGTGCTCACTCG |
1 |
1 |
100 |
0.091 |
0.091 |
| 34 |
CS-19
mAoR35 |
F-CTTTCGTTCCAATGCTCCTC
R-TGTGACAGTTCGGCTGTT |
2 |
1 |
50 |
0.259 |
0.259 |
| 35 |
CS-20
mAoR41 |
F-GCTTAGCCGGCACGATATTA
R-AGCTCACCTCGTTTCGTTTC |
3 |
3 |
100 |
0.373 |
1.119 |
| 36 |
CS-10
mAoR42c |
F-ACTGTCACGTCAATGGCATC
R-GCGAAGGGTCAAAGAGCAGTC |
1 |
1 |
100 |
0.133 |
0.133 |
| 37 |
CS-17
mAoR44 |
F-CACGTTCGCATCATCCAA
R-CGTCAGAGATTACGGCATTG |
2 |
2 |
100 |
0.477 |
0.954 |
| 38 |
|
Total/Mean |
33 |
31 |
93.3 |
0.323 |
0.747 |
| 39 |
|
Overall Total/mean |
196 |
160 |
81.6 |
0.281 |
1.318 |
| *PIC-Polymorphic information content; MI-Marker index |