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Hindi Journal of Veterinary Science & Medical DiagnosisISSN: 2325-9590
Ravina Author
Subjects of specialization
Affiliation
Animal Genetics and Breeding
Center for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India
Biography
Mr. Ravina, belongs to the department of animal science, interest in the field of Marek’s disease virus; Genosensor; Poultry; Herpesvirus; Amperpmetric; Gold electrode, from Maharshi Dayanand University, Rohtak, Haryana, India
Publications
Research Article Open Access
An Amperometric Genosensor for Detection of Virus Causing Marek’s Disease in Poultry
Author(s): Poonam Nandal, Rahul khatri, Vishakha Aggarwal,Ravina,Minakshi Prasad,Hari Mohan and Chandra S. Pundir
The 5’-thiolated DNA probe of Meq oncogenic gene consisting of 24 deoxynucleotide, was reduced by Dithiothreitol (DTT) and immobilized covalently via thiolated bond onto gold electrode followed by hybridization with 1-4 ng/μl of Marek’s Disease Virus (MDV) Single-Stranded (ss) DNA probe from tumor sample of chicken for 10 min at room temp. The Meqgenosensor could detect 1 ngss-DNA in tumor samples in min by Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV) and Electrochemical Impedance (EI). The ssDNA modified Au electrode was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). An amperometric genosensor was constructed for detection of Meq gene by connecting ssDNA electrode modified Au electrode (working ele... view more»
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