In vitro Accumulation of Active Metabolites in Physalis peruviana (Linn.) Callus

Physalis peruviana (Linn.) is a medicinal plant which is widely used in folk medicine as anticancer, antimycobacterial, antipyretic, immunomodulatory and for treating diseases such as malaria, asthma, hepatitis, dermatitis, diuretism and rheumatism. In vitro studies with Physalis peruviana were carried out through tissue culture. The calluses raised from leaf explants on MS medium supplemented with different concentrations of PGRs were compared with their explant after four and eight weeks. Callus raised on MS medium + 2% sucrose supplemented with 4.0mg/L NAA and 1.0mg/L BA exhibited significant callus growth upto eight weeks with high amount of ascorbic acid accumulation, total protein content and peroxidase activity as compared to the explants, indicating the possibility of its further usage for high secondary metabolite isolation than from the explants.