International Publisher of Science, Technology and Medicine

VEGETOS: An International Journal of Plant Research

Research Article

Screening and Bioactivity Measurement of High altitude Medicinal Plants of Nepal

Deepak Sharma*, Tara Shrestha, Naresh Rapal, Satiz C Poudel, Sujan Shrestha, Surekshya Sharma and Janardan Lamichane
Department of Biotechnology, Kathmandu University, Dhulikhel, Kavre, Nepal
Corresponding author : Deepak Sharma
Department of Biotechnology, Kathmandu University, Dhulikhel, Kavre, Nepal
E-mail: deepakshrm59@gmail.com
Received: July 27, 2016 Accepted: September 29, 2016 Published: September 30, 2016
Citation: Sharma D, Shrestha T, Rapal N, Poudel SC, Shrestha S, et al. (2016) Screening and Bioactivity Measurement of High altitude Medicinal Plants of Nepal. Vegetos 29:4. doi:10.4172/2229-4473.1000172

Abstract

Screening and Bioactivity Measurement of High altitude Medicinal Plants of Nepal

Ten different plants collected from various area of Khaptad National Park, one of the famous National Parks renowned for the source of medicinal herb located at an altitude from 1,000 m to 3,300 m, P. edgeworthii, B. petiolaris, E. strobilifera were found to have potent cytotoxicity effect while three others, T. foliolosum, B. diffusa and A. spectabilis with LC50 of 64.50, 13.59, 6.14 respectively showed very strong cytotoxicity effect against brine shrimp nauplii. The DPPH scavenging assay of all those plants was found to vary from very high to low. B. petiolaris (13.49%) showed almost the same scavenging activity to that of Ascorbic acid (17.20%) followed by E. strobilifera (6.35%) and H. salicifolia (2.38%) at a concentration of 0.5 µg/ml. The antimicrobial activity against five microorganisms (Bacillus cereus, Bacillus subtillis, Klebsiella pneumonia, Bacillus thuringiensis and Enterobacter), showed a remarkable Zone Of Inhibition (ZOI). H. salicifolia showed 6.5 mm ZOI against Bacillus cereus, 7 mm against Bacillus thuringiensis and 6 mm against Klebsiella pneumonia at 25µg/ml whereas A. spectabilis showed 9 mm as highest ZOI against Enterobacter at 25 µg/ml.

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