Some Phytochemical Studies and TLC/HPTLC method for Bark and Seed Extracts of Holarrhena antidysenterica Wall.

Some Phytochemical Studies and TLC/HPTLC method for Bark and Seed Extracts of Holarrhena antidysenterica Wall.

Present study deals with the phytochemicals of Holarrhena antidysenterica which are of great therapeutic importance. Holarrhena antidysenterica, known to be a powerful medicinal plant has been studied well for the bioactive compounds present in the leaf, bark, seed and roots. In present study, bark and seeds were investigated for phytochemicals. Dry powder of bark and seeds was used for qualitative analysis of phytochemicals and they were found to contain alkaloids, carbohydrates, amino acids, phenols, tannins, cardiac glycosides, saponins, flavonoids, terpinoids, steroids, fixed oils and fats. The main steroidal alkaloid present in Holarrhena antidysentrica is conessine which is the main bioactive compound. Analysis of conessine is performed on TLC aluminium plate pre-coated with silica gel 60 F-254 as stationary phase. Scanning and analysis of plate was carried out in absorption range 200-800 nm. This study involves preliminary phytochemical screening, the separation and identification of compounds present in the crude extract of bark and seed of Holarrhena antidysenterica by TLC and HPTLC method. The presence of peaks in the TLC and HPTLC exhibited the presence of principle components in the methanolic extracts of bark and seed.