Identification and Utility of SRAP Markers Linked to Bacterial Wilt Resistance Gene in Potato

Identification and Utility of SRAP Markers Linked to Bacterial Wilt Resistance Gene in Potato

Bacterial wilt caused by Ralstonia solanacearum is one of the most economically important diseases affecting potato (Solanum tuberosum). It is necessary to develop more molecular markers for potential use in potato genetic research. A highly resistant primitive cultivated species S. phureja was employed to generate a F1 mapping population to perform the bulked segregant analysis (BSA) for screening and identifying of sequence related amplified polymorphism (SRAP) markers linked to the potato resistance to bacterial wilt. A linkage map containing 23 DNA markers distributed on 3 linkage groups, and covering a genetic distance of 111 cM with an average distance of 5.8 cM between two markers was developed. Two SRAP markers, Me2em5 linked in repulsion phase and Me2em2 in coupling phase, flanked the resistance gene at genetic distances of 3.5 and 3.7cM distance, respectively. These markers and two others were used for early seedling selection in a BC1 population. The results showed that this marker system could be used in marker assisted selection (MAS) breeding program.