Age Dependent Changes of Biological Properties in the Multipotent Mesenchymal Stromal Bone Marrow Cells in FVB Mice
Objective: In the last years researchers’ attention has been given to the immunomodulatory properties of multipotent mesenchymal stromal cells. The question of effectiveness of the stem cell therapy with MSCs from elderly donors is worth studying. Our aim was to compare the potency of bone marrow MSCs in aging.
Methods: BM-MSCs were obtained using the standard methods. The following methods were applied: immunophenotyping, colony-forming unit fibroblast assay, granulocyte/macrophage colony-forming cells assay in the semi-agar cultures, directed differentiation, colorimetric method, MTT assay.
Results: The stromal progenitor cells increase in their number indicating age-associated elevated ability of the BM-MSCs to proliferation. The BM-MSCs have shown their ability for osteogenic and adipogenic differentiation along with age-associated abnormalities in the osteogenic differentiation. The BM-MSCs expressed immunomodulatory effect in dose-dependent manner regardless the donor age.
- The obtained culture cells derived from adult and old mice according to phenotypic profile and capacity for directed multilineage differentiation meet minimum criteria for the MSCs.
- Age-dependent differences of directed osteogenic BM-MSC differentiation have been shown.
- BM MSCs regardless of mouse age expressed a dosedependent inhibitory effect on the mitogen-induced proliferation of splenocytes.