VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078

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Antioxidant and Anti-Cholinergic Activities of Phenolic Compounds Isolated From Thymus Linearis Collected from Dir, Pakistan

Antioxidant and Anti-Cholinergic Activities of Phenolic Compounds Isolated From Thymus Linearis Collected from Dir, Pakistan

Due to the presence of polyphenol with strong natural antioxidant activity, thyme has been considered as a dietary supplement for many centuries. The main goal of the study was carried out to explore the phenolic compounds from Thymus linearis collected form Dir, Pakistan along with its antioxidant activity. From the methanolic extract of Thymus linearis, six compounds were isolated and identified as p-cymene, oleanolic acid, luteolin (LN), 4,5 dicaffeoyl quinic acid (DCQA), rosmarinic acid (RA) and salvianolic acid (SA) by column chromatography method. Antioxidant activity of isolated compounds by DPPH method, superoxide radical scavenging and ABTS assay as well as anticholinergic activity were measured with different concentrations which showed the results significantly. Data obtained from results of antioxidant and anticholinergic assays showed that IC50 values different doses were in order as DCQA>SA>RA>LN. So the study reveals that Thymus linearis contained phenolic compounds having strong natural antioxidant activity even at low dose (p<0.05). So it can be suggested that by adding the thymus in our daily life food, oxidative stress related disease can be combated due to its strong antioxidant activity..

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