Biomarkers in Drug Development#

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Biochemical Response of Sea bass Dichentrarchus labrax Experimentally Exposed to Nickel and Chlorpyriphos

Biochemical Response of Sea bass Dichentrarchus labrax Experimentally Exposed to Nickel and Chlorpyriphos

Despite the large number of studies that have investigated the relationship between metals exposure and the oxidative stress biomarkers in several marine organisms, few studies were interested to toxic effect of Ni and Chlorpyriphos mixture on the disturbance of the antioxidant system and AChE activity in fish species. The effect of nickel (Ni), Chlorpyriphos (CPF) and their mixture on oxidative stress biomarkers and on brain acetylcholinesterase activity (AChE) was investigated in sea bass D. labrax under laboratory-controlled conditions in a 7-day exposure. Fishes were exposed to 500 μg/kg Ni, 10 mg/Kg CPF or to their mixture. At 0, 1, 3 and 7 days of exposure, catalase and lipid peroxidation level were determined in liver and acetylcholinesterase activity was measured in brain of treaded and relative controls fishes. Catalase activity (CAT) and lipoperoxydation level (LPO) were significantly enhanced by Ni, CPF and their mixture and the maximum increases were observed at day 7. The increased activity of CAT and LPO level in sea bass compared to control values indicates that these animals are facing an oxidative challenge. In the case of AChE activity it was reduced only by CPF exposure, while the mixture CPF and Ni highly stimulate this activity displaying neurotoxicity to the sea bass. Dicentrarchus labrax could be regarded as a suitable bioindicator of Ni, CPF exposure by measuring CAT and AChE activities and lipid peroxidation level as biomarkers.

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