VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

https://blogum.blogaaja.fi/
https://blogum-1.jimdosite.com/
https://blogummm.edublogs.org/
https://blogummm.websites.co.in/
https://blogum18.wordpress.com/
https://benim-blogum.jigsy.com/
https://fuiegs-symbeaurds-build.yolasite.com/
https://blogum-03.webselfsite.net/
https://blogummm.mystrikingly.com/
https://blogum.splashthat.com/
https://blogum3.webnode.com.tr/
https://blogum.odoo.com/
https://blogum.creatorlink.net/
https://whiteseotr1-s-site.thinkific.com/enrollments
https://blogum.estranky.cz/
https://653ba4fbb538c.site123.me/
https://blogum12m.blogspot.com/
https://blogum.hashnode.dev/
https://whiteseoturkey1.wixsite.com/blogum
https://sites.google.com/view/blogummm/
https://codepen.io/blogum
https://blogumm.livejournal.com/
https://wakelet.com/@blogum82816
https://www.homify.com/users/9538383/blogum
https://lessons.drawspace.com/profile/323613/blogum
https://my.desktopnexus.com/blogum/
https://writeupcafe.com/profile/BLOGUM/
https://www.pearltrees.com/blogum
https://www.easyfie.com/blogum
https://pharmahub.org/members/27615/profile
https://www.zupyak.com/u/blogum/posts
https://www.metroflog.co/blogum
https://www.fuzia.com/fz/blogum-blogum
https://tr.pinterest.com/blogum12/
https://my.getjealous.com/blogum
https://micro.blog/blogum
https://www.tumblr.com/blogummm
https://hub.docker.com/u/blogum
https://fire.blogfree.net/?act=Profile&MID=1342323
https://blogum.pixnet.net/blog
https://www.threadless.com/@blogum/activity
https://blogum.neocities.org/
https://blogum12.amebaownd.com/
https://teletype.in/@blogum
https://ubl.xml.org/users/blogum
https://educatorpages.com/site/blogum/
https://blogum.onlc.fr/

Fast Protocol For Extraction of DNA From Arid Adapted Prosopis Leaves Without Liquid Nitrogen

Fast Protocol For Extraction of DNA From Arid Adapted Prosopis Leaves Without Liquid Nitrogen

The extraction of high quality genomic DNA for PCR amplification from Prosopis sp., is complicated due to the presence of a high percentage of secondary metabolites which bind to or co-precipitate with nucleic acids. In the present study we report a modified sodium dodecyl sulfate/phenol protocol that includes elimination of liquid nitrogen in maceration process, β-mercaptoethanol in buffer extraction and ethanol precipitation step. The A260/280 absorbance ratios of isolated DNA were around 2.0 to 1.9, suggesting that the DNA fraction was pure and may be used for further PCR analysis. The DNA isolated by this protocol is of sufficient quality for molecular applications; this technique could be applied to other organisms that have similar substances that hinder DNA extraction. Finally, this proposal represents an alternative fast, cheap, and effective for isolation of genomic DNA from fresh leaves of Prosopis spp., even in low technology laboratories .

Special Features

Full Text

View

Track Your Manuscript

Media Partners

GET THE APP