VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Selection of Culture Media and Optimization of Media Composition for Extracellular Production of Lipase by Acinetobacter sp. AH-19

Selection of Culture Media and Optimization of Media Composition for Extracellular Production of Lipase by Acinetobacter sp. AH-19

A total of 20 strains of bacteria were isolated from cow shade area soil collected in India. The isolated strains were screened using Rhodamine B dye on tributyrin containing nutrient agar petriplates. One isolated strain, exhibiting a broader clear zone than the remaining ones, indicating its higher lipase activity, was selected and identified as Acinetobacter sp. AH-19 based on morphological and biochemical characterization. Out of the four culture media (MI to M-IV) tested, the maximum production (78 U/ml) of lipase was observed in M-IV, that consisted of (% w/v): starch (0.8); peptone (1.0); NH4Cl (0.5); MgSO4 (0.05); CaCl2 (0.06); K2HPO4 (0.09); olive oil (0.2 % v/v) and tween-80 (0.2 % v/v). Optimization of media composition of M-IV for the production of lipase was performed by one factor at a time approach and the maximum production of lipase was observed at (% w/v): starch (0.4) 87 U/ ml , peptone (0.8) 110 U/ml, NH4Cl (1.0) 114.5 U/ml, MgSO4 (0.02) 121 U/ml, CaCl2 (0.1) 129 U/ml, K2HPO4 (0.05) 135 U/ml, Olive oil (0.25 % v/v) 149 U/ml and tween-80 (0.25 % v/v) 158 U/ml. .

Special Features

Full Text

View

Track Your Manuscript

Share This Page

Media Partners

Associations