VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Standardization of Sterilization Procedure for In Vitro Propagation of Capegooseberry (Physalis peruviana L)

For in vitro propagation of cape gooseberry (Physalis peruviana L.) explants were properly disinfected in order to obtain noncontaminated cultures. All the steps in standardization of micro propagation protocol was carried out in such a way as there was no chance of any contamination throughout in-vitro culture process. The explants taken from field were sterilized before their inoculation in sterile growth medium to remove dirt and dust adhering to the explants. Sodium hypochlorite (NaOCl), was tested for sterilization of nodal segments with varying times of exposure. Immersion of explants in sodium hypochlorite solution (1%) for 5minutes showed maximum survival (83.00%). Maximum mortality (46.00%) was observed when sodium hypochlorite (1%) was used for 8 minutes.

Special Features

Full Text

View

Track Your Manuscript

Share This Page

Media Partners

Associations