Journal of Plant Physiology & Pathology ISSN: 2329-955X

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Research Article, J Plant Physiol Pathol Vol: 3 Issue: 1

Antioxidative Capacity and Electrolyte Leakage in Healthy Versus Phytoplasma Infected Tissues of Euphorbia coerulescens and Orbea gigantea

Dewir YH1,2*, El Mahrouk ME2,3, Hafez YM4, Rihan HZ3, Sáez CA5,6 and Fuller MP3
1Plant Production Department, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi Arabia
2Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
3School of Biological Sciences, Faculty of Science and Technology, University of Plymouth, Drake Circus, PL4 8AA, United Kingdom
4Department of Agricultural Botany, Plant Pathology and Biotechnology Laboratory, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
5Departmento de Medio Ambiente, Facultad de Ingenieria Universidad de Playa Ancha, Casilla 34-v, Valparaiso, Chile
6Avanzados, Universidad de Playa Ancha, Traslavina #450, Vina del Mar, Chile
Corresponding author : Dewir YH
Plant Production Department, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi Arabia
E-mail: [email protected]
Received: September 13, 2014 Accepted: November 17, 2014 Published: November 22, 2014
Citation: Dewir YH, El Mahrouk ME, Hafez YM, Rihan HZ, Sáez CA, et al. (2015) Antioxidative Capacity and Electrolyte Leakage in Healthy Versus Phytoplasma Infected Tissues of Euphorbia coerulescens and Orbea gigantea. J Plant Physiol Pathol 3:1. doi:10.4172/2329-955X.1000139

Abstract

The present study reports on reactive oxygen species (ROS), antioxidant enzyme activities, electrolyte leakage and levels of abscisic acid (ABA) in healthy and phytoplasma infected (PI) tissues of Euphorbia coerulescens and Orbea gigantea. Histochemical staining for ROS indicated that PI tissues possess higher levels of hydrogen peroxide (H2O2) rather than superoxide (O2 ·-) as compared with healthy tissues in both plant species. The results indicated that superoxide dismutase (SOD) is not playing an important role in eliminating O2− in PI tissues. This was confirmed by a significantly decreased activity of SOD and a non-significant difference in O2 ·- content in PI tissues as compared to healthy tissues in both plant species. Peroxidase (POX) activity was significantly decreased while polyphenol oxidase (PPO) significantly increased in PI tissues compared with healthy tissues in both plant species. PI tissues was associated with a significant increase in catalase (CAT) and ascorbate peroxidase (APX) activity in E. coerulescens and a significant increase in glutathione reductase (GR) activity in O. gigantea. However, other antioxidant enzymes were lower compared with the healthy tissues. In both plant species, electrolyte leakage was significantly increased in PI tissues compared to healthy tissues.ABA level was decreased in PI tissues as compared to healthy tissues.

Keywords: Antioxidant enzymes; Fasciation; Euphorbia; Orbea; Phytoplasma

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