Animasaun Author

Annual Meeting Abstract Open Access

Identification and phylogenetic analysis of in vitro banana fungi contaminants based on ITS regions sequence

Author(s):

Animasaun, D. A

Plant tissue culture offer a means for the rapid production of disease-free plants in large quantities, however fungi contamination is a major constraint to its successful application. This study characterized, identified and conducted phylogenetic analysis on fungi contaminants of in vitro cultured banana based on inter-space (ITS) regions sequence. Genomic DNA were extracted from pure culture of fungi contaminants. Polymerase chain reaction amplification and Illumina short sequence were conducted using ITS1 and ITS4 primers. The nucleotide sequences were aligned for consensus and compared with NCBI GenBank using Basic Local Alignment Search Tool (BLAST). Analysis of the sequences using MEGA 7 Software at higher similarity sequence identified five Aspergillus spp., three Penicillium sp... view more»

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