Journal of Molecular Biology and Methods

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Anthony L Su Author

Subjects of specialization
gDNA, mRNA, qRT-PCR

Affiliation
Department of Environmental Health Sciences, School of Public Health, University of Michigan, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA

Biography

Myself Anthony L Su , I belong to Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029, USA. Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA


Publications

Research Article Open Access

Comparison of Rat Fetal Sex Determination Using Placental gDNA and mRNA via qRT-PCR

Author(s):

Anthony L Su and Rita Loch-Caruso

A growing need exists to consider fetal sex as a biological variable and accurately assess sex-specific effects. Among the multiple methods used to determine fetal sex, quantitative realtime polymerase chain reaction (qRT-PCR) of Sry (sexdetermining region Y) with genomic DNA (gDNA) is commonly used in addition to use of methodologies such as transcriptomics and detection of Barr body. However, Sry messenger RNA (mRNA), a product of SrygDNA, has not been previously assessed for sex determination. Using placental samples from timed-pregnant Wistar rats at gestational day (GD) 16, this study assessed the compatibility of Sry detection using gDNA versus mRNA to determine fetal sex. Samples used in this current study come from a larger study that investigated trichloroethylene (TCE) reprodu... view moreĀ»

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