Aparna MB Author

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Regulation of Glutamine Synthetase (GS) Activity and Root Morphological Plasticity in the Nitrogen Fixing Azolla- Anabaena Symbiont under Nitrogen Limiting Conditions

Author(s):

Yusuf A, Aparna MB and Heimer YM

The present study was conducted to identify the role of the endosymbiont, Anabaena azollae in fixing atmospheric N2 in the Azolla-Anabaena symbiosis. The Azolla-Anabaena azollae (association) grown in nitrogen-free modified Hoagland’s medium supplemented with 10% (v/v) N2 using an artificial gas mix, resulted in the increase in root number and root length. The GS specific activity of the association was 75 ± 6 nmole γ-glutamate mg protein-1 min-1 and the total N content was lesser in cultures grown in 10% (v/v) N2. Similarly, the artificially produced Anabaena free Azolla (endophyte-free), developed increased root length, root number and reduced GS specific activity (30 ± 10 nmole γ-glutamate mg protein-1 min-1), which on supplementation with 2.5 mM NH4NO... view more»

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