Archana Pamulaparthi Author

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In Vitro Direct Regeneration through Cotyledon Culture in Pigeon Pea [Cajanus Cajan (L.) Millsp.] and Evaluation of Genetic Fidelity using RAPD Markers

Author(s):

Mahitha Banala, Rajinikanth Marka, Archana Pamulaparthi and Rama Swamy Nanna*

In Vitro Direct Regeneration through Cotyledon Culture in Pigeon Pea [Cajanus Cajan (L.) Millsp.] and Evaluation of Genetic Fidelity using RAPD Markers

An efficient and direct in vitro regeneration of Cajanus cajan (red gram) cv ICP 26 from cotyledon explants was established. Cotyledons of 7-8 day old in vitro grown seedlings were cultured on MS/B5 medium augmented with different concentrations (0.5-5.0 mg/L) of 6-benzylaminopurine (BAP) / Kinetin (KIN). Among these concentrations, 2.0 mg/L BAP was found to be efficient in inducing maximum number of multiple shoots with 80% of response. 80% of the elongated shoots were successfully rooted on MS medium fortified with 1.0 mg/L ind... view more»

DOI: 10.5958/2229-4473.2017.00002.7

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