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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Ashwani Kumar Author
Publications
Research Article Subscription
Rapid and Efficient Regeneration Protocol in Dioscorea alata L.
Author(s): Ashwani Kumar, Monika Singh and Suresh Chand Goyal
Rapid and Efficient Regeneration Protocol in Dioscorea alata L.
We attempted to develop an improved and efficient regeneration protocol for this valuable medicinal species. Nodal explants cultured on MS medium supplemented with various concentrations of different growth hormone i.e. 2, 4-D, NAA and BAP alone or in combinations showed shoot induction or direct plantlet proliferation from the axillary buds. Highest multiplication rate (100 per cent) was obtained from nodal explants when MS medium supplemented with IBA (0.1 and 0.2 mg/l) along with BAP (2.0 mg/l), showed maximum number of plantlets 18.0±1.16 and 19.67±0.88 respectively. The in vitro raised plan... view more»