Byadgi AS Author

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Molecular Detection And Characterization of Phytoplasma Associated with Periwinkle

Author(s):

Sanjeev Kumar and Byadgi AS

Phytoplasmas are cell wall-less, non-helical prokaryotes that colonize plant phloem and insects gut wall and are known to cause devastating losses in crops and natural ecosystems worldwide. Amplification and band of ~1.8 kb and ~1.25 kb was obtained with little leaf disease of periwinkle and parthenium phyllody samples, but not other phytoplasma suspected samples with the phytoplasma specific primer pairs P1/P7 and R16F2/R2 in first and second round PCR respectively confirming its phytoplasmal etiology. The partial sequence (GenBank accession: HQ127073) of 16S rRNA gene of the present isolate shared 99 per cent homology with reported Sugarcane yellow leaf phytoplasma isolate from Brazil belonging Candidatus phytoplasma asteris (Group 16Sr I-B) isola... view more»

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