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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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D J Khandagale Author
Publications
Research Article Subscription
Regeneration Media Standardization of Pomegranate (Punica granatum L.) cv. Bhagava from Shoot tip, Cotyledonary Node, Hypocotyls and Leaves
Author(s): A A Bharose, D J Khandagale and D N Damse
Regeneration Media Standardization of Pomegranate (Punica granatum L.) cv. Bhagava from Shoot tip, Cotyledonary Node, Hypocotyls and Leaves
In this study an attempt has made to develop reliable and reproducible in vitro regeneration protocol to get healthy and well-formed plants from shoot tip, cotyledonary node hypocotyls and leaves of pomegranate cv. Bhagava for transformation and tissue culture. Shoot tips cultured on full MS+BAP (2.0 mg l-1) showed best response (70%) to shooting. Cotyledonary nodes cultured on full MS + NAA (2.0 mg l-1) + BAP (2.0 mg l-1) showed 80% shooting with 4-5 shoots per explant. Highest (60%) embryogenic callus from hypocotyl explants was indu... view more»