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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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D K Srivastava Author
Publications
Research Article Subscription
In vitro Plant Regeneration from Cotyledon and Hypocotyl Tissues of Tomato (Solanum lycopersicum L., cv. Solan Vajr)
Author(s): Poornima Sharma and D K Srivastava
In vitro Plant Regeneration from Cotyledon and Hypocotyl Tissues of Tomato (Solanum lycopersicum L., cv. Solan Vajr)
In vitro plant regeneration was assessed in tomato (Solanum lycopersicum L. cv. Solan Vajr) using cotyledon and hypocotyl explants from two weeks old aseptically grown seedlings. Callus induction was achieved by culturing cotyledon and hypocotyl explants on Murashige and Skoog (MS) medium supplemented with different combinations and concentrations of BAP, IAA, NAA, TDZ and Adenine. Adventitious shoot buds were induced from calluses/ explants by culturing on these media. Out of 54 different combinations of growth regulators (TDZ (alone), TDZ+Ad, TDZ+IAA, TDZ+... view more»