Dalya Gereige Author

Annual Meeting Abstract Open Access

Determination and characterization of tobacco mosaic virus in tobacco fields in North Lebanon

Author(s):

Dalya Gereige

The tobacco mosaic virus, of the Tobamovirus genus, has been named as the most important virus in the plant virology community. It infects cultivated plants and in particular tobacco and other members of the Solanaceae family. Our study was conducted to detect tobamovirus infections of tobacco crops in 55 regions of northern Lebanon. The detection of tobacco mosaic virus was conducted using Double Antibody Sandwich Enzyme Linked Immunosorbent assay (DAS-ELISA), and was confirmed by RT-PCR using primers that amplify different regions of the viral genome. The ELISA tests showed a positive result suggesting TMV infection in 4 tobacco samples out of 92 samples taken from Akkar and Daniye, which was confirmed by PCR using a specific primer that amplifies an 880 bp PCR product representing a ... view more»

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