differentiation of cardiosphere derived cells (CDCs) Author

Short Communication Open Access

Transforming stem cell research to cardiovascular remodeling

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Cardiovascular diseases (CVDs) remain the foremost reason of mortality and debility accounting for 31% of deaths worldwide. Currently, regeneration of damaged cardiac tissue with functional cardiomyocytes via stem cell therapy represents an effective approach in CVDs treatment. In this study, differentiation of cardiosphere derived cells (CDCs)

Cardiovascular diseases (CVDs) remain the foremost reason of mortality and debility accounting for 31% of deaths worldwide. Currently, regeneration of damaged cardiac tissue with functional cardiomyocytes via stem cell therapy represents an effective approach in CVDs treatment. In this study, differentiation of cardiosphere derived cells (CDCs) to cardiomyocytes was performed in search of enhanced cardiovascular regeneration therapeutics. Cardiac Progenitor Cells (CPCs) from rat heart were propagated by explant culture and CDCs were derived. Cardiac explant outgrowth cells (CEOCs) and CDCs were characterized by Immunofluorescence, flow cytometry and reverse transcriptase PCR. Further, CDCs were treated with an optimized concentration of 10µM 5-Azacytidine for 24 h followed by supp... view more»

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