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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Dwivedi P Author
Publications
Research Article Open Access
Regeneration Potential of Ascocentrum ampullaceum (Roxb.) Schlter through Inflorescence culture
Author(s): Shadang R, Dwivedi P and Hegde SN
Regeneration Potential of Ascocentrum ampullaceum (Roxb.) Schlter through Inflorescence culture
The paper deals with the in vitro regeneration response of immature inflorescence segments (buds, nodes and internodes) of Ascocentrum ampullaceum explants. The nutrient medium used played a key role in induction and multiplication of callus, protocorm like bodies and shoot. Inflorescence buds cultured in V&W medium supplemented with 15% coconut milk and 2% sucrose produced highest (80%) protocorm like bodies formation without any growth regulator. Inflorescence node culture in MKC medium supplemented with only 15% CM, and 15% CM and 1.0 mg l-1 NAA produced highest callus (70%) an... view more»