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Hindi Journal of Plant Physiology & PathologyISSN: 2329-955X
Edna Tanne-Sela Author
Subjects of specialization
Affiliation
Agriculture
The Robert H. Smith Faculty of Agriculture Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Biography
The Robert H. Smith Faculty of Agriculture Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel, Double-stranded RNA isolated from grapevine affected by grapevine stem-pitting disease
Publications
Research Article Open Access
A Novel Tool for Genetic Transformation in Trees
Author(s): Rita Mozes-Koch*, Edna Tanne-Sela, Ilan Sela
Genetic manipulation of trees is difficult. Using the universal vector IL-60 derived from Tomato Yellow Leaf Curl Virus (TYLCV) and auxiliary constructs, we introduced and expressed genes in grapevine, and orange trees. The genes were expressed in the trees after the constructs were introduced by direct injection into the phloem, root uptake or grafting, or in cuttings from intact plants that had been expressing the introduced genes for years. Moreover, because the disarmed virus does not support rolling-circle replication, viral progeny single-stranded DNA is not produced, IL-60 is not integrated into the plant's genome, and the construct, including the expressed gene, is not heritable. Moreover, IL-60 is not transmitted by natural insect vectors of TYLCV ... view more»
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