Eman Santali Author

Short Communication Open Access

A Comparison of Silica C and Silica Gel in Hydrophilic Interaction Mode: The Effect of Stationary Phase Surface Area

Author(s):

Eman Santali

Hydrophilic interaction chromatography (HILIC) was first described in 1990 although several interesting reviews focusing on general aspects of HILIC have been published [2-4] and it is becoming increasingly popular, (HILIC) is far from being completely understood.

To assess the effect of silica gel structure on retention in HILIC, a test system was developed using the quaternary ammonium ions benzyltrimethylammonium (BTM), benzyltriethylammonium (BTE) and benzyldimethylhexylammonium (BDM) as probes with tetramethylammonium acetate (TMA) as the counter-ion competing against the interaction of the test probes with ionised silanols in the stationary phases. Five columns were examined: 300 Å and 100 Å ACE silica gel columns, 100 Å and 60 Å Kromasil silica g... view more»

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