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Hindi Journal of Regenerative MedicineISSN: 2325-9620
Gabriela Gutierrez-Reyes Author
Subjects of specialization
Affiliation
Hepatology, Liver Cirrhosis
Department of Experimental Medicine, Laboratory of Liver, Pancreas and Motility, Faculty of Medicine, Universidad Nacional Aut
Biography
Gabriela Gutierrez-Reyes currently works at the Unit of Experimental Medicine, Universidad Nacional Autónoma de México. Gabriela does research in Gastroenterology. Their current project are 'Evaluación de la capacidad inductiva de la Matriz Colagénica sobre Células Troncales Mesenquimales Humanas hacía linaje tipo osteoblástico y tipo hepatocitos in vitro' and biomarkers of liver cirrhosis
Publications
Research Article Open Access
Human Mesenchymal Stem Cells seeded in 3D Collagen Matrix Scaffolds as a Therapeutic Alternative in Tissue Regeneration
Author(s): Benjamín León-Mancilla , Moisés Martínez-Castillo , Rocío Guerrero , Juan J Montesino , Erika Hernández-Estévez , Maria Cristina Piña-Barba , Rubén S Argüero , Gabriela Gutierrez-Reyes*
Human mesenchymal stem cells (hMSCs) are considered an ideal strategy for tissue engineering and regenerative medicine. However, their acquisition, administration route, and cell quantity are true challenges. On the other hand, the collagen scaffolds are a viable option, mimicking the extracellular matrix, utilizing collagen as the main polymer in 3D supports. The combination of hMSCs and scaffolds could enable the hMSCs to arrive at the target organ, avoiding the disadvantages of intravenous or intra-arterial therapy. We obtain and characterize MSCs from human amniotic membranes and evaluate their differentiation capacity in 3D collagen matrix scaffolds (CMSs). Their morphology, multipotency genes by RT-PCR and markers by flow cytometry were evaluated in in vitro cell cultures. The dif... view more»
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