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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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I Sreelathakumary Author
Publications
Research Article Subscription
Indirect Organogenesis in Bittergourd (Momordica charantia L.) Using Shoot tips as Explant
Author(s): J Resmi and I Sreelathakumary
Indirect Organogenesis in Bittergourd (Momordica charantia L.) Using Shoot tips as Explant
The induction of potentially organogenic callus and better proliferation of callus successfully from shoot tip explants of bittergourd was obtained either on the MS medium supplemented with IBA (4.00 mg l-1) + BA (0.50 mg l-1) + 2,4- D (2.00 mg l-1) or NAA (2.00 mg l-1) + BA (0.50 mg l-1) + 2,4- D (2.00 mg l-1) in combination with 30.00 g l-1 sucrose and 6.30 g l-1 agar level. Shoot regeneration response from shoot tip derived callus was best on MS medium fortified with 0.05 % AC + 1.00 mg l-1 BA with earlier regeneration, highest regeneration percentage, lengthy adventitious shoot a... view more»