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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Ishfaq Ahmed Hafiz Author
Publications
Research Article Subscription
Effect of Various Factors on the Efficiency of Agrobacterium-Mediated Transformation of Grape (Vitis vinifera L.)
Author(s): Maqsood Ahmed, Nadra Khan, Ishfaq Ahmed Hafiz, Nadeem Akhtar Abbasi, Muhammad Akbar Anjum and Sajjad Hussain
Effect of Various Factors on the Efficiency of Agrobacterium-Mediated Transformation of Grape (Vitis vinifera L.)
The present investigation was conducted to study the effect of inoculation time, cocultivation period and various concentrations ofhygromycin and kanamycin on genetic transformation of grape (Vitis vinifera L.) cv. King’s Ruby through Agrobacterium tumefaciens. The Chitinase gene (for fungal resistance) and GUS gene (for phenotypic expression of transgenic) were introduced in the embryogenic calli developed from leaf discs, through Agrobacterium strain LBA4404 harboring plasmid pBI121 nptII as selectable marker for GUS gene and hptII for Chitinase gene. ... view more»