Iwazaki M Author

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Three-Dimensional Reconstitution of Nerve Blood Vessel Units on Damaged Trachea and Bronchial Stump Using Hybrid-Transplantation of Skeletal muscle-derived stem cells and Bioabsorbable Polyglyconate Felt

Author(s):

Nakazato K, Tamaki T, Hirata M, Kazuno A Kohno M,Masuda R and Iwazaki M

A hybrid transplantation system using skeletal muscle-derived multipotent stem cells (Sk-MSCs) and bioabsorbable polyglyconate (PGA) felt was applied to coverage of the tracheal large-hole and the bronchial stump model, for the purpose of nerve-blood vessel supplementation to prevent tissue necrosis. Sk-MSCs were obtained from green-fluorescent transgenic (GFP-Tg) mouse muscles after 5 days of expansion culture. Experimental tracheal large-hole and bronchial stump following pneumonectomy were prepared using C57BL6N mice, and damaged portions were covered with PGA felt, which was then impregnated with expanded Sk-MSCs. At 2-10 weeks... view more»

DOI: 10.4172/2325-9620.1000123

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