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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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JA Bhat Author
Publications
Research Article Subscription
Callus Formation and Organogenesis of Tomato (Solanum lycopersicum L.)
Author(s): Rizwan Rashid, JA Bhat, ZA Bhat, W A Dar and Wajida Shafi
The experiment was conducted to develop a reproducible protocol for callus induction and in vitro regeneration of two tomato (Solanum lycopersicum L.) genotypes viz., Punjab Upma and IPA-3. For callus induction, cotyledon, leaf and hypocotyl aseptically grown seedlings tomato were used as explants. The explants were cultured on MS medium supplemented with different concentrations and combinations of hormones for callus induction and plant regeneration from callus. In both genotypes, maximum callus induction per cent was observed on MS medium supplemented with 1.0 mg/l NAA and 3.0 mg/l BAP and maximum plant regeneration was observed on MS medium supplemented IAA (1.0 mg/l) and BAP (2.0 mg/l). In Punjab Upma, the maximum per cent callus induc... view more»