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K K Nag Author
Research Article Subscription
Author(s): Anita Mehta and K K Nag
Somatic Embryogenesis and Plant Regeneration in Eleusine coracana Gaertn..
The embryogenic callus cultures of finger millet (Eleusine coracana Gaertn.) were initiated and multiplied in a medium containing 2,4-D in the range of 2.0-5.0 mgL-1. On the proliferation medium, callus differentiates localized groups of meristematic cells called proembryogenic mass on seed and mesocotyl explants and large number of globular proembryos were initiated directly on mesocotyl explants. When green nodulated seed-calli and mesocotyl calli were transferred to auxin-free medium or medium devoid of growth regulators, whole plantlet development was observed. Production of proembryogenic mass... view moreĀ»