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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Michael S Reid Author
Publications
Research Article Subscription
TRV Based Virus Induced Gene Silencing in Gladiolus (Gladiolus grandiflorus L.), A Monocotyledonous Ornamental Plant
Author(s): Alka Singh, Pushpendra Kumar, Cai-Zhong Jiang and Michael S Reid
TRV Based Virus Induced Gene Silencing in Gladiolus (Gladiolus grandiflorus L.), A Monocotyledonous Ornamental Plant
Virus-induced gene silencing (VIGS) has not yet successfully been used as a tool for gene functional analysis in non grass monocotyledonous geophytes. We therefore tested VIGS in gladiolus (Gladiolus grandiflora L) using a Tobacco Rattle Virus (TRV) vector containing a fragment of the gladiolus gene encoding phytoene desaturase (PDS). Gladiolus corms and plants were infiltrated with cultures of Agrobacterium tumefasciens transformed with the vector system. We tested the effect of different inoculum concentrations, different inoculation strategies and differ... view more»