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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Milan Prasad Author
Publications
Research Article Subscription
Phenotypic Characterization and Application of RAPD Technique in Bacillus thuringiensis isolates from North India
Author(s): Peeyush Kumar, M Abid Hussain, C S Prasad and Milan Prasad
Phenotypic Characterization and Application of RAPD Technique in Bacillus thuringiensis isolates from North India
Six strains of soil-borne bacterium, Bacillus thuringiensis, were isolated from the 79 soils sampled from different regions of Western plain zone of Uttar Pradesh, India. The strains were identified based on morphological and biochemical characterization, and further differentiated using random amplified polymorphic DNA-PCR (RAPDPCR). All strains were rod-shaped, Gram positive, with both spores and crystals, catalase positive and nitrate to nitrite reducing characteristic. Of the 25 random primers screened, only 14 primers amplified the polymorphic bands, amo... view more»