Morotomi T Author

Research Article Open Access

Effects of Polymer Encapsulated Glial Cell Line-Derived Neurotrophic Factor Secreting Cells on Odontoblast-like Cell Survival

Author(s):

Saito N, Washio A, Miyashita K, Wahlberg LU, Emerich DF, Morotomi T, Nishihara T and Kitamura C

Objective: Cells that are encapsulated into immunoprotective and implantable devices provide a means of enabling targeted, longterm, continuous, de novo synthesized delivery of very high levels of therapeutic molecules. In this study, we examined effects of polymer encapsulated glial cell line-derived neurotrophic factor (GDNF) secreting ARPE-19 cells on the in vitro viability of odontoblast-like cells (KN-3 cells) under normally lethal starvation conditions. Materials and methods: Devices (polymeric hollow fibers) were loaded with either GDNF-secreting ARPE-19 cells (GDNF-device) or non-modified ARPE-19 cells (ARPE-control device). KN-3 cell survival and function was quantified under starvation conditions when the cells were co-cultured with either control or GDNF devices. We further e... view more»

DOI: 10.4172/2325-9620.1000140

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