Nuzaiba P.M Author

Special Issue Article Open Access

Expression pattern of estrogen receptors and vitellogenin in genistein fed male and female common carp brooders

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Nuzaiba P.M

Estradiol signalling is stimulated or inhibited depending upon the interaction mechanism of estrogen receptors, ERα and ERβ. Soy-phytoestrogens, especially genistein, is known to bind these receptors and affect vitellogenin (vtg) synthesis. Fifty-six days of feeding trial was conducted to evaluate the effect of genistein on ERα, ERβ and vtg gene expression in male and female brooders of Cyprinus carpio. Five groups of fishes were fed with 0 (C), 1 (G-1), 3 (G-3), 6 (G-6) and 9 (G-9) mg% genistein contained feed during the trial. mRNA concentrations in liver tissue were determined by relative analysis using realtime PCR. The study observed a significant vtg mRNA level reduction from G-6 in female and a significant induction in male fishes after G-6. Expression of ER... view more»

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