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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Onecimo Grimaldo-Juarez Author
Publications
Research Article Subscription
Fast Protocol For Extraction of DNA From Arid Adapted Prosopis Leaves Without Liquid Nitrogen
Author(s): Claudia Yared Michel-López, Daniel González-Mendoza and Onecimo Grimaldo-Juarez
Fast Protocol For Extraction of DNA From Arid Adapted Prosopis Leaves Without Liquid Nitrogen
The extraction of high quality genomic DNA for PCR amplification from Prosopis sp., is complicated due to the presence of a high percentage of secondary metabolites which bind to or co-precipitate with nucleic acids. In the present study we report a modified sodium dodecyl sulfate/phenol protocol that includes elimination of liquid nitrogen in maceration process, β-mercaptoethanol in buffer extraction and ethanol precipitation step. The A260/280 absorbance ratios of isolated DNA were around 2.0 to 1.9, suggesting that the DNA fraction was pure and may be used for further PC... view more»