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Hindi Journal of Veterinary Science & Medical DiagnosisISSN: 2325-9590
Raymond R Rowland Author
Subjects of specialization
Affiliation
His-Tag blocking peptide, Fluorescent microsphere immunoassay, Luminex, Histidine-tagged protein, Penta-Histidine monoclonal antibody
Department of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506 USA
Biography
Raymond R Rowland, belongs to the department of Diagnostic Medicine/Pathobiology, interest in the field of Fluorescent microsphere immunoassay; Luminex; Histidine-tagged protein; Penta-Histidine monoclonal antibody; His-Tag blocking peptide; Recombinant protein; Animal viruses, from Nanjing Agricultural University,
Publications
Research Article Subscription
Application of Recombinant Histidine-Tagged Antigens in the Diagnosis of Viral Infections
Author(s): Mohammad M Hossain and Raymond R Rowland
Histidine-tagged proteins are frequently detected with antibodiesHistidine-tagged proteins are frequently detected with antibodiesspecific for the histidine tag; these are useful in immunologicalstudies. A penta-His monoclonal antibody (mAb) was used toestimate the relative amount of recombinant protein attachedto each microsphere bead set in a fluorescent microsphereimmunoassay (FMIA). In this study, the use of the anti-His mAbfor the detection of recombinant antigens from seven differentanimal viruses: porcine reproductive and respiratory syndromevirus (PRRSV), porcine circovirus type 2 (PCV2), swine influenzavirus (SIV), African swine fever virus (ASFV), classical swine fevervirus (CSFV), bovine viral diarrhea virus (BVDV), and Rift Valleyfever virus (RVF... view more»
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