Sangeeta Gupta Author

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Isolation and Identification of Defense Responsive Genes in Wheat During Incompatible Interaction with Bipolaris sorokiniana (Cochliobolus sativus) using SSH Technique

Author(s):

N Honnareddy, Rashmi Aggarwal, Sapna Sharma, Sangeeta Gupta, Gyanendra Singh, Bishnu Maya Bashya and Hemavati Ranebennur

Isolation and Identification of Defense Responsive Genes in Wheat During Incompatible Interaction with Bipolaris sorokiniana (Cochliobolus sativus) using SSH Technique

During the initial phase of host pathogen interaction, induction of defense genes is very critical for the incompatible interactions. In order to isolate the defense responsive genes during interaction of wheat with spot blotch fungus, Bipolaris sorokiniana, suppressive subtraction hybridization (SSH) technique was used. Four hundred and eighty clones were obtained from cDNA subtraction library and out of these 36% (172) were of high quality which were sequence analysed using n BLAST from NCBI database. Fo... view more»

DOI: 10.5958/2229-4473.2014.00064.0

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