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Hindi Journal of Veterinary Science & Medical DiagnosisISSN: 2325-9590
Shenghu He Author
Subjects of specialization
Affiliation
Mycoplasma bovis, Double antigen sandwich ELISA,Serodiagnostic,Analytic performance, Diagnostic performance
School of Agriculture, Ningxia University, No. 489 Helanshanxi Road, Yinchuan 750021, Ningxia Hui Autonomous Region, China
Biography
I am Shenghu He belonging to School of Agriculture, intrested in the field of Mycoplasma bovis, Double antigen sandwich ELISA, Serodiagnostic, Analytic performance, Diagnostic performance, Epidemiological performance, Prevalence from Ningxia University, 750021, Yinchuan, China
Publications
Research Article Open Access
Development and Performance Characterization of Double Antigen Sandwich ELISA for Mycoplasma bovis Antibody Detection
Author(s): Haifeng Luo, Yanan Guo, Peng Sun, Shuqiang Guo, Shenghu He, Ping Zhao, Liangbei Ke and Heping Zhang
Abstract
Background: Mycoplasma (M.) bovis is an important pathogen of cattle, which is associated with the occurrence of many. Currently there is no effective vaccine to prevent M. bovis infections, and no
commercial vaccines currently available, so the detection approach shows the importance for the control the disease and surveille the prevalence of M. bovis.
Objective: To develop and optimize an ELISA, and evaluate the analytical, diagnostic and epidemiological performance.
Animals: Field serum samples (n=368) from 9 cattle farms in different counties in Ningxia Province, China.
Methods: Different principles of antibody detection ELISA were compared, and the parameters of double antigen sandwich (DAS) ELISA with P48 recombinant antigen were optimized. All se... view more»
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