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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Shiv K Yadav Author
Publications
Research Article Subscription
An Efficient and Rapid Method of DNA Extraction For Molecular Marker Studies in Cotton (Gossypium hirsutum L.)
Author(s): Shiv K Yadav, V Vashisht, S S Gaurav, H Sindhuja, D V Singh and S K Lal
An Efficient and Rapid Method of DNA Extraction For Molecular Marker Studies in Cotton (Gossypium hirsutum L.)
The plant tissues with rigid cell wall need to be ground in liquid nitrogen for disruption of cells. Further, they are transferred to an extraction buffer containing detergents as well as reducing and chelating agents to disrupt the membranes. However, it can cause serious degradation of the genomic DNA. Moreover, the oil and phenolic compounds in cotton seeds degrade the quality and quantity of extracted DNA. The quality and purity of DNA is essential for the success of the molecular studies. Hence, it was considered worthwhile to evaluate various methods of DNA ... view more»