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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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T Prameela Devi Author
Publications
Research Article Subscription
Development of Species Specific Markers For Detection of Trichoderma Species
Author(s): T Prameela Devi, N. Prabhakaran, Deeba Kamil, Jyoti Lekha Borah and Pankaj Pandey
A set of 72 Trichoderma isolates from India were morphologically and molecularly characterized and confirmed as Trichoderma virens, T. harzianum, T. longibrachiatum and T. asperellum. ITS1 and ITS2 regions were amplified using universal ITS primers. The amplicon size varied from 550 to 600 bp in the isolates studied. Cluster analysis based on amplicon sequence of ITS region could not clearly differentiate the Trichoderma species into distinct group. Thus tef1 was used for differentiating the Trichoderma sp. The sequence analysis based on tef1 gene primers could differentiate T. longibrachiatum, T. virens, T. harzianum and T. asperellum species and the phylogenetic analyses were in conformity with the morphological observat... view more»