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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Vivekanand P Rao Author
Publications
Research Article Subscription
Optimization of Protocol for Embryogenic Callus induction and Regeneration of Saccharum officinarum L. var. Co 0239
Author(s): Vivekanand P Rao, R S Sengar, Vinay Sharma1 and S K Bhatnagar
Optimization of Protocol for Embryogenic Callus induction and Regeneration of Saccharum officinarum L. var. Co 0239
An efficient embryogenesis and direct regeneration protocol for commercial sugarcane cultivar Co 0239 was optimized to obtain embryogenic callus and direct regeneration of plantlets from immature leaf discs. Callus induction was achieved on Murashige and Skoog’s (MS) medium supplemented with 2, 4-dichlorophenoxy acetic acid (2,4 D) in combination with kinetin, abscisic acid, proline, maltose, sucrose and coconut water. Medium containing 2, 4-D (4 mg/l), maltose (3 %), proline (500 mg/l), kinetin (1.5 mg/l) induced 80% embryogenic callus while MS medium... view more»